egf-tm7.htm

EGF-TM7 family proteins

Saskia Neudek, Rachel Abbott & Pietro Roversi in collaboration with Dr. P. Handford (Department of Biochemistry) and Prof. S. Gordon (Sir William Dunn School of Pathology)

Lea Group

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egf The EGF-TM7 family is a group of cell-surface molecules characterised by a unique chimaeric structure in which tandem EGF (Epidermal Growth Factor-like) repeats are coupled to a G-protein coupled receptor moiety via a mucin-like stalk. They are implicated in a range of biological function but are or particular interest to us due to the identification of one of these proteins (CD97) as a T-cell ligand for CD55. To date we have grown crystals of a natural variant of CD97 termed EMR2.

We have Ba, Sr and Ca crystal forms (although the symmetry varies) and are currently producing SeMet labelled protein – a combination of data from these crystals should allow phasing of this structure. In the future we hope to study other isoforms of this molecule and a complex between CD97 and CD55

Crystallisation and preliminary X-ray diffraction analysis of three EGF domains of EMR2, a 7-TM immune system molecule.

Abbott et al., Acta Cryst, D60, 936-938

Molecular analysis of the epidermal growth factor-like short consensus repeat domain-mediated protein-protein interactions: dissection of the CD97-CD55 complex.
Lin et al., J Biol Chem. 2001 Jun 29;276(26):24160-9