picornavirus.htm

Picornavirus-Receptor Interactions
Pietro Roversi and David Pettigrew with Dr. D. Brown (University of Cambridge), Dr. D.J. Evans (University of Glasgow) and Dr. D. Bhella (University of Glasgow)

Lea Group

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Structures

Picornaviruses and their receptors have been much studied by the crystallographic community. We are using surface plasmon resonance, cryo-electron microscopy and X-ray crystallography to attempt to better understand the varied ways in which enteroviruses use CD55 to aid cell-entry.

Interaction of Decay Accelerating Factor (DAF) with hemagglutinating human enteroviruses: Utilising variation in primate DAF to map virus binding sites.
Williams et al. , J. Gen. Virol., 85(3), 731-738

The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay accelerating factor (CD55).
Bhella et al., J. Biol. Chem. 2004 Feb 27;279(9):8325-8332. [PDB ID: 1upn ]

Model of the equine rhinitis A virus capsid: identification of a major neutralizing immunogenic site.
Kriegshauser et al. , J Gen Virol. 2003 Sep;84(Pt 9):2365-2373.

Determination of the structure of a decay accelerating factor-binding clinical isolate of echovirus 11 allows mapping of mutants with altered receptor requirements for infection.
Stuart et al., J Virol. 2002 Aug;76(15):7694-704. [PDB ID: 1h8t ]

Determination of the affinity and kinetic constants for the interaction between the human virus echovirus 11 and its cellular receptor, CD55.
Lea et al., J Biol Chem. 1998 Nov 13;273(46):30443-7.