Laboratory of Molecular Biophysics Laboratory Journal 2000 SKP1/SKP2/CDK2/cyclin A complex

Figure 3. (a) Gel filtration chromatogram (labelled A) showing the purification of the SKP1/SKP2(1-153)/CDK2/cyclin A complex. Peak A1= SKP1/SKP2(1-153)/pCDK2/cyclin A3, peak A2=pCDK2/cyclin A3 and peak A3 = pCDK2 and cyclin A3. For comparison, SKP1/SKP2(1-153) (run B), and pCDK2/cyclin A3 (run C) were run separately. Peak A2=C1. CDK2/cyclin A undergoes slight dissociation in the buffer conditions used for run A (peak A3). The SKP1/SKP2(1-153) complex when not associated with pCDK2/cyclin A3 runs as an apparent dimer (peak B1).

(b) SDS-PAGE analysis of fractions from run A. Lanes 1 and 2 contain the quarternary complex (peak A1). This peak tails into the peak of excess pCDK2/cyclin A3 in the mixture, (peak A2, lanes 5 and 6). Lanes 7 and 8, peak A3.

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