Laboratory of Molecular Biophysics Laboratory Journal 2000 SKP1/SKP2/CDK2/cyclin A complex

Figure 4. pCDK2/cyclin A3 phosphorylation of GST-Rb and SKP2(1-153). The GST-Rb fusion is the major radiolabelled band in lanes 1-3. SKP2(1-153) runs towards the bottom of the gel in lanes 4-6.

• Lane 1: GST-Rb with suc1 peptide;
• Lane 2: GST-Rb with p107c peptide;
• Lane 3: GST-RB with no added peptide;
• Lane 4: SKP1/SKP2(1-153)/CDK2/cyclin A3 with no added peptide;
• Lane 5: SKP1/SKP2(1-153)/CDK2/cyclin A3 with suc1 peptide;
• Lane 6: SKP1/SKP2(1-153)/CDK2/cyclin A3 with p107c peptide.

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